Deadline for applications: 4 September 2023
Suggested starting date: late 2023
Duration: 2 to 3 years
Suggested starting date: late 2023
Duration: 2 to 3 years
We are looking for a research scientist/engineer to spearhead the development of new genetic tools, via classic transgenesis or CRISPR KI, in the crustacean Parhyale.
Background – Our team studies leg regeneration in the crustacean Parhyale hawaiensis using a combination of transgenic, live imaging and molecular profiling approaches (see Averof 2022, Paris et al. 2022). We increasingly rely on transgenic approaches to identify and study cis-regulatory elements, to mark diverse cell populations for live imaging, and to manipulate gene expression in a conditional and cell/tissue-specific manner. These efforts require streamlining existing transgenesis and CRISPR KI approaches to generate transgenic lines more efficiently and on a larger scale. The recruited developer/engineer will spearhead this effort, both as a manager and a hands-on experimenter.
Activities – The recruited person will become familiar with the microinjection, transgenesis and CRISPR methods that have already been established in Parhyale. S/he will then establish an efficient transgenesis/CRISPR pipeline, including streamlined procedures for microinjection, screening for transformants, and establishing transgenic lines. S/he will manage the transgenesis infrastructure and collaborate closely with other team members to help them implement their transgenesis/CRISPR experiments. In parallel to supporting team projects, the developer/engineer will have opportunities to develop his/her own projects, testing new tools and generating transgenic lines of broad interest (e.g. cell/tissue-specific markers, Brainbow-like tools for mosaic labelling, tools for conditional cell ablation). S/he will author publications on the methods s/he develops and the projects to which s/he contributes.
Work environment – The transgenic tools developer/engineer will be fully integrated in the Averof lab (averof-lab.org), participating in lab meetings, seminars, lab retreats and training courses. S/he will collaborate closely with the team leader and other team members (postdocs, PhD students, technicians) to provide support, discuss and prioritise objectives. The lab’s working language is English.
The post is funded for 2-3 years by a grant from the Fondation pour la Recherche Médicale. It could be extended further, depending on additional funding.
Activities – The recruited person will become familiar with the microinjection, transgenesis and CRISPR methods that have already been established in Parhyale. S/he will then establish an efficient transgenesis/CRISPR pipeline, including streamlined procedures for microinjection, screening for transformants, and establishing transgenic lines. S/he will manage the transgenesis infrastructure and collaborate closely with other team members to help them implement their transgenesis/CRISPR experiments. In parallel to supporting team projects, the developer/engineer will have opportunities to develop his/her own projects, testing new tools and generating transgenic lines of broad interest (e.g. cell/tissue-specific markers, Brainbow-like tools for mosaic labelling, tools for conditional cell ablation). S/he will author publications on the methods s/he develops and the projects to which s/he contributes.
Work environment – The transgenic tools developer/engineer will be fully integrated in the Averof lab (averof-lab.org), participating in lab meetings, seminars, lab retreats and training courses. S/he will collaborate closely with the team leader and other team members (postdocs, PhD students, technicians) to provide support, discuss and prioritise objectives. The lab’s working language is English.
The post is funded for 2-3 years by a grant from the Fondation pour la Recherche Médicale. It could be extended further, depending on additional funding.
Expected qualifications –
* Experience in microinjection, transgenesis and/or CRISPR approaches
* Experience in cell or developmental biology would be an advantage, but not essential
* Organisational and interpersonal/collaborative skills
* Communication in English
* Experience in microinjection, transgenesis and/or CRISPR approaches
* Experience in cell or developmental biology would be an advantage, but not essential
* Organisational and interpersonal/collaborative skills
* Communication in English
